ESTABLISHMENT AND CHARACTERIZATION OF 2 CELL-LINES DERIVED FROM HUMAN GLIOBLASTOMA-MULTIFORME

被引：0
|
作者：
BACCIOCCHI, G
GIBELLI, N
ZIBERA, C
PEDRAZZOLI, P
BERGAMASCHI, G
POLVER, PD
DANOVA, M
MAZZINI, G
PALOMBA, L
TUPLER, R
DELLACUNA, GR
机构：
[1] IST RICOVERO & CURA CARATTERE SCI FDN CLIN LAVORO,DIV ONCOL,CITOPATOL LAB,VIA S BOEZIO 26,I-27100 PAVIA,ITALY
[2] IST RICOVERO & CURA CARATTERE SCI POLICLIN S MATTEO,PAVIA,ITALY
[3] CNR,CTR STUDIO ISTOCHIM,PAVIA,ITALY
[4] UNIV PAVIA,IST ANAT COMPARATA,I-27100 PAVIA,ITALY
[5] UNIV PAVIA,IST BIOL GEN & GENET MED,I-27100 PAVIA,ITALY
来源：
ANTICANCER RESEARCH | 1992年 / 12卷 / 03期
关键词：
CELL CULTURES; GLIOMA; CHARACTERIZATION;
D O I：
暂无
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
We established and characterized two cell lines derived from glioblastoma multiforme. Both cell lines exhibited tumor cell morphology and growth kinetics and showed variable expression of glial fibrillary acidic protein (GFAP), S-100, fibronectin and vimentin. Cytofluorimetrical analysis of tumor samples showed a diploid DNA distribution, whereas permanent culture cells evolved to the hyperdiploid DNA content. Karyotype studies revealed cytogenetical abnormalities described in glial tumors including gain of chromosome 7, loss of chromosome 10 and presence of double minutes (DMs). Enhanced expression of Ha-ras and c-myc genes resulted from high p-21 and p-62 levels. The contemporary presence of TGF-alpha and EGF-Rc transcripts suggested an autocrine mechanism in the cell lines growth.
引用
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页码：853 / 861
页数：9
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