Expression of growth hormone gene during early development of Siberian sturgeon (Acipenser baerii)

被引：0
|
作者：
Abdolahnejad, Zeinab [1 ]
Pourkazemi, Mohammad [2 ]
Khoshkholgh, Majid Reza [1 ]
Yarmohammadi, Mahtab [3 ]
机构：
[1] Univ Guilan Sowmeh Sara, Fac Nat Resources, Dept Fisheries, Sowmeh Sara, Iran
[2] Iranian Fisheries Sci Res Inst, Agr Res Educ Extens, Tehran, Iran
[3] Int Sturgeon Res Inst, Rasht, Iran
来源：
MOLECULAR BIOLOGY RESEARCH COMMUNICATIONS | 2015年 / 4卷 / 04期
关键词：
Siberian sturgeon; Growth hormone; mRNA expression; RT-PCR;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The mRNA expression of growth hormone (GH) gene in early development stages of Siberian sturgeon was investigated using RT-PCR method. Samples were collected from unfertilized eggs up to 50 days post hatched (dph) larvae in 11 different times. Ribosomal protein L6 (RPL6) transcripts were used as the internal standard during quantification of GH mRNA expression. The results showed that the GH mRNA could be observed in the eyed eggs and even at unfertilized eggs of Siberian sturgeon. The highest amounts of GH mRNA were found at 25 and 50 dph larvae, while the lowest levels were detected at 1 and 3 dph larvae stage. These findings suggest that, the GH mRNA play a key role during developmental stages of Siberian sturgeon.
引用
收藏
页码：181 / 188
页数：8
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