PLANT-REGENERATION FROM STEM CORTEX EXPLANT AND PROTOPLAST CULTURES OF BRASSICA-JUNCEA (MUSTARD)

被引：0

作者：

BONFILS, AC ^{[1
]}

SPROULE, A ^{[1
]}

WEBB, JA ^{[1
]}

KELLER, WA ^{[1
]}

机构：

[1] AGR CANADA, CTR PLANT RES, OTTAWA K1A 0C6, ONTARIO, CANADA

来源：

PLANT CELL REPORTS | 1992年 / 11卷 / 12期

关键词：

BRASSICA-JUNCEA; EXPLANTS; PROTOPLASTS; REGENERATION;

D O I：

暂无

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Plant regeneration from stem cortex explants of 13 genotypes of Brassica juncea was assessed. Regeneration was strongly affected by genotype, as up to 50.6 shoots were produced per 100 calli of the most responsive line (Blaze), whereas no shoots were obtained from less responsive lines (Zem1, Vniimk351). Blaze was chosen for B. juncea stem cortex protoplast isolation. After one week of culture, 11-14 % of the cells had divided, and about 0.002 % produced 1-2 mm colonies within 6 weeks. Up to 7 % of these colonies gave rise to shoots upon transfer to plant regeneration medium.

引用

页码：614 / 617

页数：4

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