IDENTIFICATION OF PHOSPHATIDYLINOSITOL-SPECIFIC PHOSPHOLIPASE-C ACTIVITY IN LISTERIA-MONOCYTOGENES - A NOVEL TYPE OF VIRULENCE FACTOR

被引：168
|
作者：
MENGAUD, J
BRAUNBRETON, C
COSSART, P
机构：
[1] INST PASTEUR,UNITE GENIE MICROBIOL,25 RUE DR ROUX,F-75724 PARIS 15,FRANCE
[2] INST PASTEUR,UNITE PARASITOL EXPTL,F-75724 PARIS 15,FRANCE
来源：
MOLECULAR MICROBIOLOGY | 1991年 / 5卷 / 02期
关键词：
D O I：
10.1111/j.1365-2958.1991.tb02118.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A phospholipase C which cleaves phosphatidylinositol and glycosylphosphatidylinositol (GPI) anchors was identified in Listeria monocytogenes. This 36 kDa protein is encoded by the gene plcA, and is homologous to the Bacillus cereus, Bacillus thuringiensis and eukaryotic phosphatidylinositol-specific phospholipases C (PI-PLC). Expression of the plcA gene in Escherichia coli correlates with the appearance of PI-PLC activity in the cells. In Listeria monocytogenes, the activity is secreted to the culture medium. PI-PLC activity was only found in the two pathogenic species of the genus Listeria, namely L. monocytogenes and L. ivanolii. PI-PLC activity was lost and virulence decreased when the plcA gene was disrupted in the chromosome. This suggests that the PI-PLC of L. monocytogenes might be involved in virulence.
引用
收藏
页码：367 / 372
页数：6
相关论文
共 50 条
  • [31] PHOSPHATIDYLINOSITOL-SPECIFIC PHOSPHOLIPASE-C ACTIVITY OF CHROMAFFIN GRANULE-BINDING PROTEINS
    CREUTZ, CE
    DOWLING, LG
    KYGER, EM
    FRANSON, RC
    JOURNAL OF BIOLOGICAL CHEMISTRY, 1985, 260 (12) : 7171 - 7173
  • [32] INHIBITION OF PHOSPHATIDYLINOSITOL-SPECIFIC PHOSPHOLIPASE-C ACTIVITY BY FLUVIRUCIN B-2
    UI, H
    IMOTO, M
    UMEZAWA, K
    JOURNAL OF ANTIBIOTICS, 1995, 48 (05): : 387 - 390
  • [33] ANALYSIS OF A MULTIGENE FAMILY OF PHOSPHATIDYLINOSITOL-SPECIFIC PHOSPHOLIPASE-C IN SOYBEAN
    SHI, JR
    GONZALES, RA
    BHATTACHARYYA, MK
    PLANT PHYSIOLOGY, 1995, 108 (02) : 29 - 29
  • [34] PREPARATION AND APPLICATION OF AN AFFINITY MATRIX FOR PHOSPHATIDYLINOSITOL-SPECIFIC PHOSPHOLIPASE-C
    SHASHIDHAR, MS
    KEANA, JFW
    VOLWERK, JJ
    GRIFFITH, OH
    CHEMISTRY AND PHYSICS OF LIPIDS, 1990, 56 (2-3) : 159 - 167
  • [35] PHOSPHATIDYLINOSITOL-SPECIFIC PHOSPHOLIPASE-C IN FETAL MEMBRANES AND UTERINE DECIDUA
    DIRENZO, GC
    JOHNSTON, JM
    OKAZAKI, T
    OKITA, JR
    MACDONALD, PC
    BLEASDALE, JE
    JOURNAL OF CLINICAL INVESTIGATION, 1981, 67 (03): : 847 - 856
  • [36] CLONING, EXPRESSION, AND MUTAGENESIS OF PHOSPHATIDYLINOSITOL-SPECIFIC PHOSPHOLIPASE-C FROM STAPHYLOCOCCUS-AUREUS - A POTENTIAL STAPHYLOCOCCAL VIRULENCE FACTOR
    DAUGHERTY, S
    LOW, MG
    INFECTION AND IMMUNITY, 1993, 61 (12) : 5078 - 5089
  • [37] Involvement of Listeria monocytogenes phosphatidylinositol-specific phospholipase C and host protein kinase C in permeabilization of the macrophage phagosome
    Poussin, MA
    Goldfine, H
    INFECTION AND IMMUNITY, 2005, 73 (07) : 4410 - 4413
  • [38] EFFECTS OF CA++ ON PHOSPHATIDYLINOSITOL-SPECIFIC PHOSPHOLIPASE-C ACTIVITY IN RAT-BRAIN
    MIZUTA, T
    IMAI, C
    SEGAWA, T
    JAPANESE JOURNAL OF PHARMACOLOGY, 1987, 43 : P200 - P200
  • [39] SPECIFIC RELEASE OF PLASMA-MEMBRANE ENZYMES BY A PHOSPHATIDYLINOSITOL-SPECIFIC PHOSPHOLIPASE-C
    LOW, MG
    FINEAN, JB
    BIOCHIMICA ET BIOPHYSICA ACTA, 1978, 508 (03) : 565 - 570
  • [40] DIFFERENCES IN THE REGULATION OF PHOSPHATIDYLINOSITOL-SPECIFIC PHOSPHOLIPASE-C IN NORMAL AND NEOPLASTIC KERATINOCYTES
    PUNNONEN, K
    DENNING, MF
    RHEE, SG
    YUSPA, SH
    MOLECULAR CARCINOGENESIS, 1994, 10 (04) : 216 - 225
12345