MICROPLATE METHODS FOR DETERMINATION OF SERUM-CHOLESTEROL, HIGH-DENSITY-LIPOPROTEIN CHOLESTEROL, TRIGLYCERIDE AND APOLIPOPROTEINS

Microtiter plate methods were developed for the enzymatic determination of serum total cholesterol (TC), high density lipoprotein cholesterol (HDL-C) and triglyceride (TG), and for the turbidometric determination of apolipoproteins. The micromethods resulted in accurate, precise values that were in good agreement with the conventional spectrophotometric assays. The coefficient of variation for TC determinations was 4.5% or less and bias was 5% or less. The lipid micromethod assays are sensitive to 10 mg/dL or less, and the apolipoprotein assay to 1 mg/dL. Less than 100 muL of serum suffices for TC, TG and apoprotein assays; HDL-C requires an additional 100 muL of serum. Advantages of the micromethods include reductions in assay time and in the amount of reagents required.