PHORBOL ESTER INDUCES PHOSPHORYLATION AND DOWN-REGULATION OF CONNEXIN-43 IN WB-CELLS

被引：128
|
作者：
OH, SY [1 ]
GRUPEN, CG [1 ]
MURRAY, AW [1 ]
机构：
[1] FLINDERS UNIV,SCH BIOL SCI,GPO BOX 2100,ADELAIDE,SA 5001,AUSTRALIA
来源：
BIOCHIMICA ET BIOPHYSICA ACTA | 1991年 / 1094卷 / 02期
关键词：
PHORBOL ESTER; CONNEXIN-43; PHOSPHORYLATION;
D O I：
10.1016/0167-4889(91)90016-Q
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Western blot analysis indicated that WB cells contained multiple proteins of molecular mass 42-47 K which reacted with anti-connexin 43 antibody. Incubation of cells with 12-O-tetradecanoylphorbol-13-acetate (TPA) for 5 min caused the inhibition of dye transfer and the appearance of reactive material of 50 K which was abolished by acid phosphatase. Longer term incubation with TPA caused loss of connexin 43 protein.
引用
收藏
页码：243 / 245
页数：3
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