HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 GAG-POL FRAMESHIFTING IS DEPENDENT ON DOWNSTREAM MESSENGER-RNA SECONDARY STRUCTURE - DEMONSTRATION BY EXPRESSION INVIVO

被引：133

作者：

PARKIN, NT ^{[1
]}

CHAMORRO, M ^{[1
]}

VARMUS, HE ^{[1
]}

机构：

[1] UNIV CALIF SAN FRANCISCO, DEPT MICROBIOL & IMMUNOL, ROOM HSE 401, 513 PARNASSUS AVE, SAN FRANCISCO, CA 94143 USA

来源：

JOURNAL OF VIROLOGY | 1992年 / 66卷 / 08期

关键词：

D O I：

10.1128/JVI.66.8.5147-5151.1992

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The human immunodeficiency virus type 1 (HIV-1) Gag-Pol fusion polyprotein is produced via ribosomal frameshifting. Previous studies in vitro and in Saccharomyces cerevisiae have argued against a significant role for RNA secondary structure 3' of the shift site, in contrast with other systems, in which such structure has been shown to be required. Here we show, by expressing the HIV-1 gag-pol domain in cultured vertebrate cells, that a stem-loop structure 3' of the HIV-1 shift site is indeed important for wild-type levels of frameshifting in vivo.

引用

页码：5147 / 5151

页数：5

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