CRYSTALLIZATION AND PRELIMINARY-X-RAY ANALYSIS OF A LIPASE FROM STAPHYLOCOCCUS-HYICUS

被引：8
|
作者：
RANSAC, S
BLAAUW, M
DIJKSTRA, BW
SLOTBOOM, AJ
BOOTS, JWP
VERHEIJ, HM
机构：
[1] UNIV GRONINGEN,BIOPHYS CHEM LAB,9747 AG GRONINGEN,NETHERLANDS
[2] UNIV GRONINGEN,BIOSON,RES INST,DEPT CHEM,9747 AG GRONINGEN,NETHERLANDS
[3] UNIV UTRECHT,CTR BIOMEMBRANES & LIPID ENZYMOL,3508 TB UTRECHT,NETHERLANDS
来源：
JOURNAL OF STRUCTURAL BIOLOGY | 1995年 / 114卷 / 02期
关键词：
D O I：
10.1006/jsbi.1995.1014
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Single crystals of a lipase from Staphylococcus hyicus have been obtained using a combination of 18 to 24% dimethylsulfoxide and 10% isopropanol as a precipitant. The crystals grow at 4 degrees C in 2-3 months. They belong to the orthorhombic space group P2(1)2(1)2(1) with a = 73.31 Angstrom, b = 77.96 Angstrom, and c = 169.81 Angstrom, with two protein molecules per asymmetrical unit. The crystals diffract to at least 2.8 Angstrom resolution and are suitable for an X-ray structure analysis. (C) 1995 Academic Press,lnc.
引用
收藏
页码：153 / 155
页数：3
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