IDENTIFICATION AND LOCALIZATION OF THE FIRST GLUTAREDOXIN IN LEAVES OF A HIGHER-PLANT

被引：34

作者：

MORELL, S ^{[1
]}

FOLLMANN, H ^{[1
]}

HABERLEIN, I ^{[1
]}

机构：

[1] UNIV KASSEL,FACHBEREICH BIOL CHEM,D-34109 KASSEL,GERMANY

来源：

FEBS LETTERS | 1995年 / 369卷 / 2-3期

关键词：

SPINACH LEAVE; GLUTAREDOXIN; THIOREDOXIN; LOCALIZATION;

D O I：

10.1016/0014-5793(95)00690-B

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Glutaredoxin(thioltransferase) has been identified and purified to homogeneity from spinach leaves. Its cytosolic localization was demonstrated by chromatographic and immunological analysis of extracts from isolated spinach chloroplasts and mitochondria, respectively. Spinach glutaredoxin shows a significant crossreactivity with antibodies raised against E. coli glutaredoxin and possesses a specific thioltransferase activity comparable to that of the E. coli protein. Minor thioltransferase activities (less than 10% of total leaf activity) have been observed in spinach chloroplasts which are probably due to the presence of trypsin inhibitor and thioredoxins (TRf and TRm).

引用

页码：149 / 152

页数：4

共 50 条

[1] FLUORESCENCE OF HIGHER-PLANT LEAVES AT RAISED TEMPERATURES
KUZNETSOVA, EA
[J]. BIOFIZIKA, 1982, 27 (05): : 809 - 811
[2] PRESENCE OF FREE-IRON IN HIGHER-PLANT LEAVES
ALIEV, DI
KURBANOV, IS
VANIN, AF
[J]. STUDIA BIOPHYSICA, 1986, 115 (03): : 173 - 180
[3] IMMUNOGOLD LOCALIZATION OF PHYTOENE DESATURASE IN HIGHER-PLANT CHLOROPLASTS
LINDEN, H
LUCAS, MM
DEFELIPE, MR
SANDMANN, G
[J]. PHYSIOLOGIA PLANTARUM, 1993, 88 (02) : 229 - 236
[4] TARGETING OF GLYOXYSOMAL PROTEINS TO PEROXISOMES IN LEAVES AND ROOTS OF A HIGHER-PLANT
OLSEN, LJ
ETTINGER, WF
DAMSZ, B
MATSUDAIRA, K
WEBB, MA
HARADA, JJ
[J]. PLANT CELL, 1993, 5 (08): : 941 - 952
[5] MAGNETIC MODULATION OF FLUORESCENCE INTENSITY IN HIGHER-PLANT LEAVES INVIVO
TRIBEL, MM
KOLESNIKOVA, LI
FRANKEVICH, EL
[J]. DOKLADY AKADEMII NAUK SSSR, 1981, 257 (04): : 1020 - 1024
[6] LOCALIZATION AND IDENTIFICATION OF PHOSPHOPROTEINS WITHIN THE PHOTOSYSTEM-II CORE OF HIGHER-PLANT THYLAKOID MEMBRANES
MILLNER, PA
MARDER, JB
GOUNARIS, K
BARBER, J
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1986, 852 (01) : 30 - 37
[7] STRATEGIES FOR THE IDENTIFICATION AND ANALYSIS OF HIGHER-PLANT MITOCHONDRIAL GENES
DAWSON, AJ
JONES, VP
LEAVER, CJ
[J]. METHODS IN ENZYMOLOGY, 1986, 118 : 470 - 485
[8] IDENTIFICATION OF THE ALTERNATIVE TERMINAL OXIDASE OF HIGHER-PLANT MITOCHONDRIA
ELTHON, TE
MCINTOSH, L
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (23) : 8399 - 8403
[9] BIOTIN BIOSYNTHESIS IN HIGHER-PLANT CELLS - IDENTIFICATION OF INTERMEDIATES
BALDET, P
GERBLING, H
AXIOTIS, S
DOUCE, R
[J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1993, 217 (01): : 479 - 485
[10] DETECTION AND SUBCELLULAR-LOCALIZATION OF A HIGHER-PLANT CHORISMATE SYNTHASE
MOUSDALE, DM
COGGINS, JR
[J]. FEBS LETTERS, 1986, 205 (02) : 328 - 332

← 1 2 3 4 5 →