RELEASE OF CALCITONIN GENE-RELATED PEPTIDE-LIKE (CGRP-LI) IMMUNOREACTIVITY FROM RAT ISOLATED SOLEUS MUSCLE BY LOW PH, CAPSAICIN AND POTASSIUM

被引：32
|
作者：
SANTICIOLI, P
DELBIANCO, E
GEPPETTI, P
MAGGI, CA
机构：
[1] A MENARINI PHARMACEUT,DEPT PHARMACOL,VIA SETTE SANTI 3,I-50131 FLORENCE,ITALY
[2] UNIV FLORENCE,DEPT INTERNAL MED,I-50121 FLORENCE,ITALY
来源：
NEUROSCIENCE LETTERS | 1992年 / 143卷 / 1-2期
关键词：
CGRP; PROTON; RAT SOLEUS MUSCLE; CAPSAICIN-SENSITIVE PRIMARY AFFERENT; RUTHENIUM RED;
D O I：
10.1016/0304-3940(92)90223-T
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
Capsaicin (10-mu-M), KCl (80 mM) or superfusion with a low pH medium (pH 5 or 6) produced a significant increase of calcitonin gene-related peptide-like immunoreactivity (CGRP-LI) outflow from the superfused rat isolated soleus muscle. CGRP-LI outflow produced by capsaicin or pH 5 medium was totally abolished in a calcium free medium containing EDTA (1 mM) and the effect of pH 5 medium was prevented by a previous application of capsaicin. Ruthenium red (10-mu-M) produced a marked inhibition of CGRP-LI release produced by capsaicin or pH 5 medium (69 and 84%, resp.), without affecting that evoked by KCl. These findings demonstrate that protons activate capsaicin-sensitive primary afferents in rat skeletal muscle through a Ruthenium red-sensitive mechanism. Proton-induced CGRP-LI release in skeletal muscle could be of relevance during exercise and/or skeletal muscle ischemia.
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页码：19 / 22
页数：4
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