PLATELET DENSE GRANULE RELEASE REACTION MONITORED BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY FLUOROMETRIC-DETERMINATION OF ENDOGENOUS SEROTONIN

被引：23
|
作者：
ANDERSON, GM [1 ]
HALL, LM [1 ]
YANG, JX [1 ]
COHEN, DJ [1 ]
机构：
[1] YALE UNIV, SCH MED, DEPT LAB MED, NEW HAVEN, CT 06510 USA
来源：
ANALYTICAL BIOCHEMISTRY | 1992年 / 206卷 / 01期
关键词：
D O I：
10.1016/S0003-2697(05)80011-9
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The platelet dense granule release reaction was monitored by measuring the serotonin content of platelets collected by filtration following release. Sensitive HPLC-fluorometric analysis permitted release to be assessed using small (25-50 μl) volumes of plasma and whole blood. Release could be examined for periods as short as 1 s due to the rapid stoppage effected by filtration. The half-life of thrombin-stimulated (1 U/ml) release was 2-3 s, the EC50 values observed for thrombinstimulated release over a 60-s release period were 0.01-0.03 U/ml for diluted whole blood and plasma samples. © 1992 Academic Press, Inc.
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页码：64 / 67
页数：4
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