PURIFICATION AND PARTIAL CHARACTERIZATION OF 2 LECTIN ISOFORMS FROM CRATYLIA-MOLLIS MART (CAMARATU BEAN)

被引：64
|
作者：
PAIVA, PMG [1 ]
COELHO, LCBB [1 ]
机构：
[1] UNIV FED PERNAMBUCO,CTR CIENCIAS BIOL,DEPT BIOQUIM,AV MORAES REGO S-N,CIDADE UNIV,BR-50730 RECIFE,PE,BRAZIL
来源：
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 1992年 / 36卷 / 02期
关键词：
LECTIN; MULTIPLE MOLECULAR FORMS;
D O I：
10.1007/BF02929691
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Two additional electrophoretically distinct molecular forms, isoforms (iso) 2 and 3, with lectin properties were isolated from Cratylia mollis Mart. seeds (FABACEAE), by extraction with 0.15M NaCl and ammonium sulfate fractionation, followed by chromatography on Sephadex G-75 and Bio-Gel P-200 (iso 2), as well as CM-Cellulose and Sephadex G-75 (iso 3). Both isoforms were human group nonspecific and showed distinct specificity. Polyacrylamide gel electrophoresis resolved iso 2 and 3 in polypeptides of apparent mol wts 60 and 31 kDa, respectively; a distinct isoelectric focusing pattern was obtained for iso 2 and 3, under denaturing and reducing conditions.
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收藏
页码：113 / 118
页数：6
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