IDENTIFICATION OF RESIDUES INVOLVED IN POLYMORPHIC ANTIBODY-BINDING EPITOPES ON HLA-DR MOLECULES

被引:10
|
作者
FU, XT
YU, WY
ALBER, C
BENSON, C
WATTS, R
NORDWIG, H
JOHNSON, JP
KNOWLES, RW
KARR, RW
机构
[1] UNIV IOWA,DEPT VET AFFAIRS MED CTR,COLL MED,540 EMRB,IOWA CITY,IA 52242
[2] UNIV IOWA HOSP & CLIN,DEPT INTERNAL MED,IOWA CITY,IA 52242
[3] UNIV MUNICH,INST IMMUNOL,W-8000 MUNICH 2,GERMANY
[4] MEM SLOAN KETTERING CANC CTR,NEW YORK,NY 10021
关键词
D O I
10.1016/0198-8859(92)90051-N
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Based on previous studies it was predicted that amino acids 4 or 25 of the DR4-beta-1 and DR7-beta-1 chains are involved in polymorphic antibody binding epitopes on DR4 or DR7 molecules. These predictions were tested by analyzing monoclonal antibody (mAb) binding to transfectants expressing mutant DR4-beta-1 or DR7-beta-1 chains with single amino acid substitutions at positions 4 or 25. Antibody binding to transfectants expressing additional DR4/7-beta-1 hybrids was also analyzed to assess further the contributions of four segments of the DR4-beta-1 or DR7-beta-1 chains: amino acids 1-20, 21-40, 41-97, and the beta(2) domain. Single amino acid substitutions at positions 4 and 25 of the DR4-beta-1 chain or DR7-beta-1 chain eliminate binding of several mAb to DR4 or DR7 molecules, documenting that these residues are involved in antibody epitopes. However, the data with the hybrid DR4/7-beta-1 chains indicate that some of these epitopes require contributions from both segments 1-20 and 21-40 of these DR-beta chains, whereas other epitopes can be generated by placing the appropriate segment in the context of the other DR-beta chain. In addition, the data with other mAb indicate that their epitopes are determined primarily by sequences within the 41-97 segment or in the beta(2) domain.
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页码:47 / 56
页数:10
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