CONSTRUCTION OF ACTINOBACILLUS-PLEUROPNEUMONIAE ESCHERICHIA-COLI SHUTTLE VECTORS - EXPRESSION OF ANTIBIOTIC-RESISTANCE GENES

被引：39

作者：

WEST, SEH ^{[1
]}

ROMERO, MJM ^{[1
]}

REGASSA, LB ^{[1
]}

ZIELINSKI, NA ^{[1
]}

WELCH, RA ^{[1
]}

机构：

[1] UNIV WISCONSIN,SCH MED,DEPT MED MICROBIOL & IMMUNOL,MADISON,WI 53706

来源：

GENE | 1995年 / 160卷 / 01期

关键词：

PASTEURELLA HAEMOLYTICA; CLONING; GENE EXPRESSION;

D O I：

10.1016/0378-1119(95)00236-Y

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We constructed several cloning vectors, designated pGZRS-18/19 and pGZRS-38/39, which were based on an endogenous Actinobncillus pleuropneumoniae (Apl) 4.3-kb plasmid. They carry the lacZ alpha-complementation fragment and MCS from pUC18/19, and either the bla gene under the control of a putative Apl promoter or the Km(R) gene from Tn903. These vectors replicate in representative strains of Apl serotypes 1 and 7, Escherichia coli, Pasteurella haenzolytica (Ph) and Haemophilus (Actinobacillus) actinomycetemcomitans. We also found that Apl and Ph did not express genes under the control of the lacZ or bla promoters, suggesting that their RNA polymerases may not utilize these promoters.

引用

页码：81 / 86

页数：6

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