GENETIC-ANALYSIS OF THE FLAA LOCUS OF BACILLUS-SUBTILIS

被引:12
|
作者
HAUSER, PM
CRABB, WD
FIORA, MG
SCOFFONE, F
GALIZZI, A
机构
[1] UNIV PAVIA,DIPARTIMENTO GENET & MICROBIOL A BUZZATITRAVERSO,I-27100 PAVIA,ITALY
[2] CNR,IST GENET BIOCHIM & EVOLUZ,I-27100 PAVIA,ITALY
[3] UNIV LAUSANNE,INST GENET & BIOL MICROBIENNES,CH-1005 LAUSANNE,SWITZERLAND
[4] GENENCOR INC,S SAN FRANCISCO,CA 94080
关键词
D O I
10.1128/jb.173.11.3580-3583.1991
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
We isolated two clones of recombinant lambda bacteriophage with overlapping inserts of Bacillus subtilis chromosomal DNA corresponding to part of the flaA locus. The flaA4 and flaA15 mutations were localized on the physical map by marker rescue experiments. The flaA locus and the flaB (sigD) gene were mapped in transduction crosses, and the order glnA polC flaB flaA was determined. FlaB was linked to polC in transformation crosses.
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页码:3580 / 3583
页数:4
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