CIRCADIAN OSCILLATION OF 64-KDA POLYPEPTIDE IN THE RAT SUPRACHIASMATIC NUCLEUS

被引:0
|
作者
NISHI, R [1 ]
MURAKAMI, N [1 ]
MARUMOTO, N [1 ]
NASU, T [1 ]
KURODA, H [1 ]
ETOH, T [1 ]
机构
[1] MIYAZAKI UNIV, FAC AGR, DEPT VET ANAT, MIYAZAKI 89921, JAPAN
来源
JOURNAL OF VETERINARY MEDICAL SCIENCE | 1994年 / 56卷 / 01期
关键词
D O I
10.1292/jvms.56.161
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
In cell suspensions of suprachiasmatic nucleus harvested every 3 hr from rats kept under 12 hr: 12 hr light-dark cycle and constant darkness, we have detected a Mr 64-kDa protein whose synthesis exhibits two distinct daily peaks in SDS-PAGE. Analysis of densitometer tracings revealed that the synthesis of other proteins was independent of the time of day or not reproducible. Maximum synthesis of the 64-kDa polypeptide occurred at around CT6 and CT21, which are almost coincident with the phase advance regions of circadian activity rhythm induced by anisomycin and light pulses [15], respectively. These results suggest that the 64-kDa protein in SDS-PAGE may be a part of the circadian clock mechanism.
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页码:161 / 163
页数:3
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