PRIMARY STRUCTURE OF THE CDNA 5'-TERMINAL REGION ENCODING THE N-TERMINAL DOMAIN OF THE RABBIT MUSCLE ALPHA-ACTININ SUBUNIT

被引：5
|
作者：
JOKHADZE, GG [1 ]
OLEINIKOV, AV [1 ]
ALAKHOV, YB [1 ]
NADIRASHVILI, NS [1 ]
ZAALISHVILI, MM [1 ]
机构：
[1] ACAD SCI GESSR,INST MOLEC BIOL & BIOL PHYS,TBILISI 380060,GEORGIA,USSR
来源：
FEBS LETTERS | 1991年 / 289卷 / 02期
关键词：
ALPHA-ACTININ; RABBIT SKELETAL MUSCLE; 96-FOLD DEGENERATE OLIGONUCLEOTIDE MIX; AMV REVERSE TRANSCRIPTASE STRONG STOP SIGNAL;
D O I：
10.1016/0014-5793(91)81067-I
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A number of cDNA clones have been obtained in summary encoding the N-terminal domain containing 286 amino acid residues of the rabbit skeletal muscle alpha-actinin subunit. Alpha-actinin cDNA clones were isolated from specific cDNA libraries using the primer extension method for synthesis of the first cDNA chain. A strong stop signal for AMV reverse transcriptase in the 5'-terminal region of mRNA of alpha-actinin was found. It seems there is a G+C rich region (93% G+C nucleotides) including a continuous sequence of 23 G and C nucleotides encoding 6 glycine residues.
引用
收藏
页码：190 / 192
页数：3
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