SECRETION OF SINGLE-CHAIN UROKINASE-TYPE PLASMINOGEN-ACTIVATOR FROM INSECT CELLS

被引：7
|
作者：
KING, LA [1 ]
KAUR, K [1 ]
MANN, SG [1 ]
LAWRIE, AM [1 ]
STEVEN, J [1 ]
OGDEN, JE [1 ]
机构：
[1] DELTA BIOTECHNOL LTD,NOTTINGHAM NG7 1FD,ENGLAND
来源：
GENE | 1991年 / 106卷 / 02期
关键词：
BACULOVIRUS; AUTOGRAPHA-CALIFORNICA NUCLEAR POLYHEDROSIS VIRUS; POLYHEDRON PROMOTER; SPODOPTERA-FRUGIPERDA CELLS; RECOMBINANT DNA;
D O I：
10.1016/0378-1119(91)90194-G
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
A cDNA encoding human urokinase-type plasminogen activator was inserted downstream from the polyhedrin promoter of the baculovirus Autographa californica nuclear polyhedrosis virus. A protein of similar M(r) to urokinase (UK) was synthesized and approx. 90% was secreted from recombinant virus-infected Spodoptera frugiperda cells. Zymography and Western blotting analysis of the insect-derived protein demonstrated that it was comprised solely of the high-M(r) form of UK. No low-M(r) UK was detected. Amidolytic activity assays showed that 96% of the insect cell-derived UK was in the single-chain proenzyme form. The yield of UK from insect cells was 1986 international units/Ml/10(6) infected cells.
引用
收藏
页码：151 / 157
页数：7
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