DEMONSTRATION OF ANTIBODIES AGAINST BOVINE LEUKEMIA-VIRUS (BLV) BY BLOCKING ELISA USING BOVINE POLYCLONAL ANTI-BLV IMMUNOGLOBULIN

被引：10

作者：

HAVE, P ^{[1
]}

HOFFJORGENSEN, R ^{[1
]}

机构：

[1] NATL VET LAB,DK-1503 COPENHAGEN V,DENMARK

来源：

VETERINARY MICROBIOLOGY | 1991年 / 27卷 / 3-4期

关键词：

D O I：

10.1016/0378-1135(91)90149-A

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A blocking enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies against bovine leukemia virus (BLV) is described. The test is based on the biotin-streptavidin system using unlabelled polyclonal bovine IgG against BLV as catching antibody and biotinylated bovine anti-BLV IgG as detecting antibody. The sensitivity was found to be 50-100 times higher than the agar gel immunodiffusion test, with a specificity of practically 100%. The blocking ELISA proved to be suitable for detection of antibodies against BLV in serum and milk. In 34 paired milk/serum samples, the average ratio of BLV antibody titres was 1 : 26. So far, more than 700 000 sera have been screened by blocking ELISA for BLV antibodies in the course of the Danish surveillance programme for BLV infection.

引用

页码：221 / 229

页数：9

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