CALPAIN IN CULTURED BOVINE LENS EPITHELIAL-CELLS

被引：11
|
作者：
LIPMAN, RD
CYR, DE
DAVID, LL
TAYLOR, A
机构：
[1] TUFTS UNIV,USDA,HUMAN NUTR RES CTR AGING,NUTR VIS RES LAB,711 WASHINGTON ST,BOSTON,MA 02111
[2] OREGON HLTH SCI UNIV,SCH DENT,DEPT BIOCHEM,PORTLAND,OR 97201
[3] OREGON HLTH SCI UNIV,SCH MED,DEPT OPHTHALMOL,PORTLAND,OR 97201
来源：
CURRENT EYE RESEARCH | 1991年 / 10卷 / 01期
关键词：
D O I：
10.3109/02713689109007606
中图分类号：
R77 [眼科学];
学科分类号：
100212 ;
摘要：
Calcium dependent proteolysis was examined in supernatant prepared from cultured bovine lens epithelial (BLE) cells. The presence of the calcium activated protease, calpain, was indicated by immunorecognition of 80 kDa and 30 kDa subunits of calpain in BLE cell supernatant. Degradation of I-125-alpha-crystallin and FITC labeled casein by BLE cell supernatant were shown to be calcium dependent. Inhibition of activity was achieved with EGTA, calpastatin or CbzValPheH. The data presented are the first measurement of calpain activity in cultured lens cells.
引用
收藏
页码：11 / 17
页数：7
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