BREFELDIN-A AFFECTS WEST NILE VIRUS-REPLICATION IN VERO CELLS BUT NOT C6/36 CELLS

被引：15

作者：

SREENIVASAN, V ^{[1
]}

NG, KL ^{[1
]}

NG, ML ^{[1
]}

机构：

[1] NATL UNIV SINGAPORE,DEPT MICROBIOL,KENT RIDGE,SINGAPORE 0511,SINGAPORE

来源：

JOURNAL OF VIROLOGICAL METHODS | 1993年 / 45卷 / 01期

关键词：

BREFELDIN-A; FLAVIVIRUS; GLYCOPROTEIN;

D O I：

10.1016/0166-0934(93)90135-E

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A fungal metabolite brefeldin A (BFA) was used to study virus-host interaction in glycoprotein processing in West Nile virus-infected Vero and C6/36 cells. The results indicated that as little as 1 mug/ml of BFA resulted in complete breakdown in the Golgi organelle in infected Vero cells. This led to modifications of the glycoproteins which could not be efficiently used in infectious virion formation. In contrast, as much as 10 mug/ml of BFA in culture medium did not affect either glycoprotein formation or production of infectious particles in C6/36 cells. The results showed that in Vero cells, the transport of glycoproteins to the Golgi apparatus is important in West Nile virus infection. It also showed that BFA could be used as a tool to understand further the trafficking of glycoprotein from the ER to Golgi in flavivirus infection in Vero cells.

引用

页码：1 / 17

页数：17

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