B-SUBUNIT OF CHOLERA-TOXIN PRODUCED IN ESCHERICHIA-COLI

被引：0
|
作者：
MA, QJ
LIU, CX
XIONG, LS
YU, XQ
机构：
来源：
SCIENCE IN CHINA SERIES B-CHEMISTRY LIFE SCIENCES & EARTH SCIENCES | 1991年 / 34卷 / 03期
关键词：
ESCHERICHIA-COLI; CHOLERA TOXIN;
D O I：
暂无
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
An engineered E. coli strain containing high expression level of CT-B subunits has been obtained by the application of recombinant DNA techniques. The B subunit can be secreted into the medium and reaches 20-40-mu-g/ml when this strain is incubated in a 50 l fermentation tank. The CT-B subunit purified with affinity chromatography in E. coli has the same characters as the natural CT-B subunit in molecular weight, N terminal amino acid analysis and antigenicity. The CT-B subunit has good immunogenicity and can be used as a preparation for protecting against diarrhea caused by V. cholera and enterotoxigenic E. coli. It can also be used as a vector for hepatins.
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页码：274 / 280
页数：7
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