CDNA LIBRARIES FROM HUMAN TISSUES AND CELL-LINES

被引：18
|
作者：
SWAROOP, A
XU, J
机构：
[1] UNIV MICHIGAN,DEPT OPHTHALMOL,ANN ARBOR,MI 48105
[2] UNIV MICHIGAN,DEPT HUMAN GENET,ANN ARBOR,MI 48105
来源：
CYTOGENETICS AND CELL GENETICS | 1993年 / 64卷 / 3-4期
关键词：
D O I：
10.1159/000133595
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
We report the construction of 34 cDNA libraries from human tissues and cell lines in lambda phage vectors (Charon BS, lamdbagt11, lambdaSHK, or lamdazapII). The cDNA was synthesized from poly A+ RNA, using oligo-dT as a primer, and size-selected before ligation to vector arms. High-complexity cDNA libraries from human tissues and cell lines should be a valuable resource for genome mapping studies and identification of disease genes.
引用
收藏
页码：292 / 294
页数：3
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