Enhanced Detection of Glycans by MALDI-TOF Mass Spectrometry Using a Binary Matrix of 2,5-Dihydroxybenzoic Acid and 2,6-Dihydroxybenzoic Acid

被引：0
|
作者：
Kim, Yunjin [1 ]
Kim, Taehee [1 ]
Lee, Jihyeon [1 ]
Im, Haeju [1 ]
Kim, Jeongkwon [1 ]
机构：
[1] Chungnam Natl Univ, Dept Chem, Daejeon, South Korea
来源：
MASS SPECTROMETRY LETTERS | 2013年 / 4卷 / 02期
基金：
新加坡国家研究基金会;
关键词：
Glycans; MALDI; PNGase F; ovalbumin; oligosaccharides; dihydroxybenzoic acid;
D O I：
10.5478/MSL.2013.4.1.38
中图分类号：
O433 [光谱学];
学科分类号：
0703 ; 070302 ;
摘要：
Glycans released from ovalbumin by PNGase F were analyzed by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry using three different dihydroxybenzoic acid (DHB) matrix systems: 2,5-DHB, 2,6-DHB, and a 2,5-DHB/2,6-DHB binary matrix. Relative to the results obtained with the single-component matrices (2,5-DHB or 2,6-DHB), the 2,5-DHB/2,6-DHB binary matrix boasted lower background noise and higher sensitivity. A total of 16 glycan peaks were observed using the 2,5-DHB/2,6-DHB binary matrix, while only 10 and 9 glycan peaks were observed using the 2,5-DHB and 2,6-DHB matrices, respectively.
引用
收藏
页码：38 / 40
页数：3
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