Enhanced Detection of Glycans by MALDI-TOF Mass Spectrometry Using a Binary Matrix of 2,5-Dihydroxybenzoic Acid and 2,6-Dihydroxybenzoic Acid

被引：0
|
作者：
Kim, Yunjin [1 ]
Kim, Taehee [1 ]
Lee, Jihyeon [1 ]
Im, Haeju [1 ]
Kim, Jeongkwon [1 ]
机构：
[1] Chungnam Natl Univ, Dept Chem, Daejeon, South Korea
来源：
MASS SPECTROMETRY LETTERS | 2013年 / 4卷 / 02期
基金：
新加坡国家研究基金会;
关键词：
Glycans; MALDI; PNGase F; ovalbumin; oligosaccharides; dihydroxybenzoic acid;
D O I：
10.5478/MSL.2013.4.1.38
中图分类号：
O433 [光谱学];
学科分类号：
0703 ; 070302 ;
摘要：
Glycans released from ovalbumin by PNGase F were analyzed by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry using three different dihydroxybenzoic acid (DHB) matrix systems: 2,5-DHB, 2,6-DHB, and a 2,5-DHB/2,6-DHB binary matrix. Relative to the results obtained with the single-component matrices (2,5-DHB or 2,6-DHB), the 2,5-DHB/2,6-DHB binary matrix boasted lower background noise and higher sensitivity. A total of 16 glycan peaks were observed using the 2,5-DHB/2,6-DHB binary matrix, while only 10 and 9 glycan peaks were observed using the 2,5-DHB and 2,6-DHB matrices, respectively.
引用
收藏
页码：38 / 40
页数：3
相关论文
共 50 条
  • [1] Dyeing polymorphs: The MALDI host 2,5-dihydroxybenzoic acid
    Cohen, Dawn E.
    Benedict, Jason B.
    Morlan, Brian
    Chiu, Daniel T.
    Kahr, Bart
    CRYSTAL GROWTH & DESIGN, 2007, 7 (03) : 492 - 495
  • [2] Use of a 2,5-dihydroxybenzoic acid/aniline MALDI matrix for improved detection and on-target derivatization of glycans: A preliminary report
    Snovida, Sergei I.
    Chen, Vincent C.
    Perreault, Helene
    ANALYTICAL CHEMISTRY, 2006, 78 (24) : 8561 - 8568
  • [3] 2,5-DIHYDROXYBENZOIC ACID - A NEW MATRIX FOR LASER DESORPTION IONIZATION MASS-SPECTROMETRY
    STRUPAT, K
    KARAS, M
    HILLENKAMP, F
    INTERNATIONAL JOURNAL OF MASS SPECTROMETRY AND ION PROCESSES, 1991, 111 : 89 - 102
  • [4] PROTONATION AND DIMERIZATION OF 2,4-DIHYDROXYBENZOIC, 2,5-DIHYDROXYBENZOIC, 2,6-DIHYDROXYBENZOIC AND 3,5-DIHYDROXYBENZOIC ACIDS IN 0.5M SODIUM-PERCHLORATE MEDIUM
    LAJUNEN, LHJ
    SAARINEN, J
    PARHI, S
    TALANTA, 1980, 27 (01) : 71 - 74
  • [5] 2,6-DIHYDROXYBENZOIC ACID ANILIDES AS FASCIOLICIDES
    DUWEL, D
    METZGER, H
    JOURNAL OF MEDICINAL CHEMISTRY, 1973, 16 (05) : 433 - 436
  • [6] MALDI Mass Spectrometric Analysis of Nonderivatized Steroids Using Cyclodextrin-supported 2,5-Dihydroxybenzoic Acid as Matrix
    Son, Jeongjin
    Cha, Sangwon
    BULLETIN OF THE KOREAN CHEMICAL SOCIETY, 2014, 35 (05): : 1409 - 1412
  • [7] 2,5-Dihydroxybenzoic acid solution in MALDI-MS: ageing and use for mass calibration
    Teearu, Anu
    Vahur, Signe
    Haljasorg, Uku
    Leito, Ivo
    Haljasorg, Toiv
    Toom, Lauri
    JOURNAL OF MASS SPECTROMETRY, 2014, 49 (10): : 970 - 979
  • [8] THE DISSOCIATION OF SALICYLIC, 2,4-DIHYDROXYBENZOIC, 2,5-DIHYDROXYBENZOIC, AND 2,6-DIHYDROXYBENZOIC ACIDS IN METHANOL WATER MIXTURES AT 25-DEGREES-C
    POULIAS, G
    PAPADOPOULOS, N
    JANNAKOUDAKIS, D
    ZEITSCHRIFT FUR NATURFORSCHUNG SECTION A-A JOURNAL OF PHYSICAL SCIENCES, 1983, 38 (12): : 1402 - 1404
  • [9] Measurement and Correlation of the Solubility of 2,6-Dihydroxybenzoic Acid in Alcohols and Binary Solvents
    Wang, Yuyan
    Chen, Yanxin
    Zhu, Peipei
    Bao, Ying
    Xie, Chuang
    Gong, Junbo
    Jiang, Xiaobin
    Hou, Baohong
    Chen, Wei
    JOURNAL OF CHEMICAL AND ENGINEERING DATA, 2017, 62 (10): : 3009 - 3014
  • [10] 2,6-DIHYDROXYBENZOIC ACID-DERIVATIVES AS ANTHELMINTICS
    RUSCHIG, H
    KONIG, J
    DUWEL, D
    LOEWE, H
    ARZNEIMITTEL-FORSCHUNG/DRUG RESEARCH, 1973, 23 (12): : 1745 - 1758
12345