INTERLEUKIN-4 AND TUMOR-NECROSIS-FACTOR-ALPHA INDUCE DIFFERENT ADHESION PATHWAYS IN ENDOTHELIAL-CELLS FOR THE BINDING OF PERIPHERAL-BLOOD LYMPHOCYTES

被引:23
|
作者
GALEA, P [1 ]
LEBRANCHU, Y [1 ]
THIBAULT, G [1 ]
BARDOS, P [1 ]
机构
[1] UNIV TOURS,FAC MED,CHU BRETTONNEAU,IMMUNOL LAB,2 BIS BLVD TONNELLE,F-37032 TOURS,FRANCE
关键词
D O I
10.1111/j.1365-3083.1992.tb03226.x
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
We demonstrated that tumour necrosis factor alpha (TNF-alpha) and interleukin 4 (IL-4) increased endothelial cell (EC) adhesiveness for peripheral blood lymphocytes (PBL) by promoting transcription and protein synthesis. The different kinetics observed with TNF-alpha and IL-4 suggest the involvement of different adhesion molecules. Blocking adhesion assays and immunofluorescence analysis showed that PBL adhesion to endothelial cells involves different pair adhesion molecules. Whereas IL-4 promoted an LFA-1-dependent/ICAM-1-independent adhesion pathway on EC, TNF-alpha stimulated an LFA-1-dependent/ICAM-1-dependent adhesion pathway on EC. In contrast, VLA-4/VCAM-1 molecules were involved in PBL adhesion both to IL-4 and to TNF-alpha-stimulated EC. Finally, we found that a CD2-dependent/LFA-3-independent adhesion pathway was mainly involved in IL-4-stimulated EC.
引用
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页码:575 / 585
页数:11
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