ELECTROPHORETIC KARYOTYPING OF THE LIGNIN-DEGRADING BASIDIOMYCETE PHANEROCHAETE-CHRYSOSPORIUM

被引:7
|
作者
DSOUZA, TM [1 ]
DASS, SB [1 ]
RASOOLY, A [1 ]
REDDY, CA [1 ]
机构
[1] MICHIGAN STATE UNIV,DEPT MICROBIOL,E LANSING,MI 48824
关键词
D O I
10.1111/j.1365-2958.1993.tb01627.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Electrophoretic karyotyping of the two most widely studied strains of Phanerochaete chrysosporium, BKMF-1767 and ME-446, has been determined using transverse alternating field electrophoresis. The genomic DNA of BKMF-1767 was resolved into 10 chromosomes ranging in size from 1.8-5.0 Mb, amounting to a total genome size of about 29 Mb. The genomic DNA of strain ME-446, on the other hand, was resolved into 11 chromosomes, amounting to a total genome size of about 32 Mb. Lignin peroxidase genes have been localized to five chromosomes in strain BKMF-1767 and to four chromosomes in strain ME-446.
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页码:803 / 807
页数:5
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