EXPRESSION OF TETRAHYMENA ACTIN IN MAMMALIAN-CELLS

被引：3
|
作者：
HIRONO, M [1 ]
SAIJOKURITA, K [1 ]
KURASHIMA, J [1 ]
WATANABE, Y [1 ]
OHNO, T [1 ]
机构：
[1] UNIV TSUKUBA,INST BIOL SCI,TSUKUBA,IBARAKI 305,JAPAN
来源：
CELL BIOLOGY INTERNATIONAL REPORTS | 1992年 / 16卷 / 07期
关键词：
D O I：
10.1016/S0309-1651(06)80006-8
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
We previously revealed that Tetrahymena actin can copolymerize with rabbit skeletal muscle actin whereas it has a very divergent primary structure and some unusual properties. To investigate the effects of coexistence of this unusual Tetrahymena actin in mammalian cells, we here transfected Tetrahymena actin gene on an expression vector into COS-1 cells. From the results of immunofluorescence microscopy, it was found that Tetrahymena actin expressed in COS-1 cells copolymerized with intrinsic actin, and it was conspicuously localized to the tips of microfilament core bundles in microspikes. On the other hand, increase in cell number tended to cease temporarily about 24 hr after transfection with Tetrahymena actin gene, implying the inhibition of cytokinesis by Tetrahymena actin coexistence. © 1992 Academic Press Ltd.
引用
收藏
页码：645 / 651
页数：7
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