INCREASE IN STABILITY OF XYLANASE FROM AN ALKALOPHILIC THERMOPHILIC BACILLUS (NCIM-59)

被引：5
|
作者：
NATH, D [1 ]
RAO, M [1 ]
机构：
[1] NATL CHEM LAB,DIV BIOCHEM SCI,POONA 411008,MAHARASHTRA,INDIA
来源：
BIOTECHNOLOGY LETTERS | 1995年 / 17卷 / 05期
关键词：
D O I：
10.1007/BF00132028
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The cellulase-free xylanase from an alkalophilic thermophilic Bacillus was stable at pH 7.0 to 10.0 at 50 degrees for 3 days. At 60 degrees the enzyme showed a decrease in stability with a half- life of 3 h. Addition of various additives had no effect on the enzyme stability at 60 degrees. Glycine (0.5M) increased-the enzyme half-life 6-fold at pH 7.0 to 9.0 and at 60 and 70 degrees. Xylan could offer protection against thermoinactivation of the xylanase at pH 7.0 and 8.0 at 60 degrees and only a marginal increase at pH 9.0 at 70 degrees was observed.
引用
收藏
页码：557 / 560
页数：4
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