CLONING AND CHARACTERIZATION OF A U6 SMALL NUCLEAR-RNA GENE FROM POTATO

被引：6

作者：

HU, YQ

BROWN, JWS

WAUGH, R

TURNER, PC

机构：

[1] UNIV LIVERPOOL,DEPT BIOCHEM,POB 147,LIVERPOOL L69 3BX,ENGLAND

[2] SCOTTISH CROP RES INST,DUNDEE DD2 5DA,SCOTLAND

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1991年 / 1129卷 / 01期

基金：

英国惠康基金;

关键词：

U6; SNRNA; SNRNA GENE; POTATO; RNA PROCESSING; SMALL NUCLEAR RNA;

D O I：

10.1016/0167-4781(91)90217-A

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Using a mixed U6 snRNA gene probe and a low stringency hybridization procedure we have isolated a U6 snRNA containing clone from a potato genomic library in lambda-EMBL 3. This clone contains a single U6 snRNA gene which has been subcloned and sequenced. Southern blotting experiments using this gene and the heterologous U6 genes as probes indicate that the potato U6 gene family consists of more than 20 members. The potato U6 gene sequence shows high identity to previously characterised plant U6 snRNA gene sequences and possesses correctly positioned and spaced transcription control elements suggesting that it is an active gene.

引用

页码：90 / 92

页数：3

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