TARGETED AND HIGHLY EFFICIENT GENE-TRANSFER INTO CD4+ CELLS BY A RECOMBINANT HUMAN-IMMUNODEFICIENCY-VIRUS RETROVIRAL VECTOR

被引：85

作者：

SHIMADA, T ^{[1
]}

FUJII, H ^{[1
]}

MITSUYA, H ^{[1
]}

NIENHUIS, AW ^{[1
]}

机构：

[1] NCI,CLIN ONCOL PROGRAM,BETHESDA,MD 20892

来源：

JOURNAL OF CLINICAL INVESTIGATION | 1991年 / 88卷 / 03期

关键词：

GENE TRANSFER; GENE THERAPY; AIDS; INTRACELLULAR IMMUNIZATION; COS CELLS;

D O I：

10.1172/JCI115365

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

We have established a recombinant HIV gene transfer system based on transient expression of the HIV packaging functions and a recombinant vector genome in monkey kidney Cos cells. The recombinant HIV retroviral vector introduced the neo(R) gene into CD4+ cells with high efficiency, comparable to that achieved with the highest titer amphotropic murine recombinant retrovirus. Vector preparations were devoid of replication competent, infectious HIV. Gene transfer was dependent on CD4 expression, as shown by expression of the CD4 gene in HeLa cells, and could be inhibited by soluble CD4. This specific and efficient gene transfer system may be useful for development of gene therapy for which T cells are the desired targets.

引用

页码：1043 / 1047

页数：5

共 50 条

[21] RETROVIRAL VECTOR-MEDIATED GENE-TRANSFER INTO ENDOTHELIAL-CELLS
DICHEK, DA
MOLECULAR BIOLOGY & MEDICINE, 1991, 8 (02) : 257 - 266
[22] INHIBITION OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 REPLICATION IN HUMAN T-CELLS BY RETROVIRAL-MEDIATED GENE-TRANSFER OF A DOMINANT-NEGATIVE REV TRANSACTIVATOR
BEVEC, D
DOBROVNIK, M
HAUBER, J
BOHNLEIN, E
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (20) : 9870 - 9874
[23] RETROVIRAL VECTOR-MEDIATED LYMPHOKINE GENE-TRANSFER INTO HUMAN RENAL-CANCER CELLS
GASTL, G
FINSTAD, CL
GUARINI, A
BOSL, G
GILBOA, E
BANDER, NH
GANSBACHER, B
CANCER RESEARCH, 1992, 52 (22) : 6229 - 6236
[24] VARIABLE STABILITY OF A SELECTABLE PROVIRUS AFTER RETROVIRAL VECTOR GENE-TRANSFER INTO HUMAN-CELLS
JOLLY, DJ
WILLIS, RC
FRIEDMANN, T
MOLECULAR AND CELLULAR BIOLOGY, 1986, 6 (04) : 1141 - 1147
[25] LYMPHOCYTES CD4+ AND OPPORTUNISTIC INFECTIONS AND NEOPLASMS IN PATIENTS WITH INFECTION BY THE HUMAN-IMMUNODEFICIENCY-VIRUS
VILLANUEVA, JL
PRADA, JL
KINDELAN, JM
TORRECISNEROS, J
MEDICINA CLINICA, 1994, 103 (19): : 758 - 759
[26] HIGHLY LOCALIZED TRACKS OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 NEF IN THE NUCLEUS OF CELLS OF A HUMAN CD4+ T-CELL LINE
MURTI, KG
BROWN, PS
RATNER, L
GARCIA, JV
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (24) : 11895 - 11899
[27] RETROVIRAL-MEDIATED GENE-TRANSFER INTO HUMAN MYELOMA CELLS
BJORKSTRAND, B
DILBER, MS
SMITH, CIE
GAHRTON, G
XANTHOPOULOS, KG
BRITISH JOURNAL OF HAEMATOLOGY, 1994, 88 (02) : 325 - 331
[28] INHIBITION OF HUMAN-IMMUNODEFICIENCY-VIRUS REPLICATION IN ACUTELY INFECTED CD4+ CELLS BY CD8+ CELLS INVOLVES A NONCYTOTOXIC MECHANISM
WALKER, CM
ERICKSON, AL
HSUEH, FC
LEVY, JA
JOURNAL OF VIROLOGY, 1991, 65 (11) : 5921 - 5927
[29] DISTURBANCE OF NUCLEAR TRANSPORT OF PROTEINS IN CD4+ CELLS EXPRESSING GP160 OF HUMAN-IMMUNODEFICIENCY-VIRUS
KOGA, Y
SASAKI, M
YOSHIDA, H
OHTSU, M
KIMURA, G
NOMOTO, K
JOURNAL OF VIROLOGY, 1991, 65 (10) : 5609 - 5612
[30] Inhibition of human immunodeficiency virus transmission to CD4+ T cells after gene transfer of constitutively expressed interferon β to dendritic cells
Cremer, I
Vieillard, V
Sautès-Fridman, C
De Maeyer, E
HUMAN GENE THERAPY, 2000, 11 (12) : 1695 - 1703

← 1 2 3 4 5 →