PHENOTYPIC ANALYSIS OF T-LYMPHOCYTES DERIVED FROM TUMOR-INFILTRATING LYMPHOCYTES AND PERIPHERAL-BLOOD MONONUCLEAR-CELLS IN PATIENTS WITH RENAL-CELL CARCINOMA

被引：0
|
作者：
ROZNOWSKI, A [1 ]
BANAT, G [1 ]
KABELITZ, D [1 ]
STAEHLER, G [1 ]
POMER, S [1 ]
机构：
[1] UNIV KLIN HEIDELBERG, INST IMMUNOL, D-69120 HEIDELBERG, GERMANY
来源：
TUMORDIAGNOSTIK & THERAPIE | 1994年 / 15卷 / 03期
关键词：
T-LYMPHOCYTES; TUMOR INFILTRATING LYMPHOCYTE; PERIPHERAL BLOOD MONONUCLEAR CELLS; RENAL CELL CARCINOMA;
D O I：
暂无
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
To study the distribution of T-lymphocytes in renal cell carcinoma, tumor-infiltrating lymphocytes (TIL) and peripheral blood mononuclear cells (PBMC) were analyzed in 31 untreated patients by two-colour flow cytometry using 33 monoclonal antibodies. T-cells from TIL and PBMC were different in CD4/CD8 ratio (1.1 vs. 2.5), and in values of activation markers: MHC-II+ (54% vs. 10 %), CD45Ro (81 % vs. 50 %), CD63 (36 % vs. 11 %) and CD69 (60 % vs. 2 %). There was a correlation between the decreasing grading and the expression of CD8, CD25, CD71 and VLA-1. However IL-2 receptor values were unexpectedly rather low (7 % vs. 4 %). The low levels of transferrine receptor (CD71) positive cells suggest an insignificant proliferation of T-lymphocytes despite their activation state. Natural killer cell markers were decreased in tumor infiltrates partly as a result of enzyme digestion, yet confirming their known deficiency in renal cell cancers.
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页码：89 / 95
页数：7
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