STABILIZATION OF THE MN-CLUSTER OF THE OXYGEN-EVOLVING COMPLEX BY GLYCINEBETAINE

被引:30
|
作者
MOHANTY, P [1 ]
HAYASHI, H [1 ]
PAPAGEORGIOU, GC [1 ]
MURATA, N [1 ]
机构
[1] NATL INST BASIC BIOL,OKAZAKI,AICHI 444,JAPAN
关键词
OXYGEN-EVOLVING COMPLEX; MANGANESE CLUSTER; PHOTOSYSTEM-II; GLYCINEBETAINE;
D O I
10.1016/0005-2728(93)90035-E
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The protective effect of glycinebetaine on inactivation of the photosynthetic oxygen evolution was studied in isolated Photosystem II particles from spinach that had been depleted of all three extrinsic proteins of the oxygen-evolving complex (ddPS2 particles). Glycinebetaine protected the ddPS2 particles against the inactivation by incubation at room temperature and at alkaline pH which may perturb the structure of the proteins that coordinate the Mn-cluster. However, glycinebetaine was not effective in protecting the ddPS2 particles against inactivation caused by incubations with NH2OH and with Mn(CH3COO)2, that are known to interact directly with the Mn-culster. It did not prevent photoinhibition of ddPS2 particles either.
引用
收藏
页码:92 / 96
页数:5
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