SYNTHESIS OF A TETRASACCHARIDE ACCEPTOR FOR USE IN THE ASSAY OF UDP-GLCPNAC - BETA-D-GALP-(1-]4)-BETA-D-GLCPNAC (GLCNAC TO GAL) BETA(1-]3)-N-ACETYLGLUCOSAMINYLTRANSFERASE ACTIVITY AND THE PENTASACCHARIDE PRODUCT THAT WOULD BE FORMED BY ITS ENZYMATIC GLYCOSYLATION

被引：9

作者：

SRIVASTAVA, G ^{[1
]}

HINDSGAUL, O ^{[1
]}

机构：

[1] UNIV ALBERTA, DEPT CHEM, EDMONTON T6G 2G2, ALBERTA, CANADA

来源：

CARBOHYDRATE RESEARCH | 1992年 / 224卷

关键词：

D O I：

10.1016/0008-6215(92)84095-A

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The tetrasaccharide beta-D-Galp-(1 --> 4)-beta-D-GlcpNAc-(1 --> 6)-alpha-D-Manp-(1 --> 6)-beta-D-Manp-OR (2) and the pentasaccharide beta-D-GlcpNAc-(1 --> 3)-beta-D-Galp-(1 --> 4)-beta-D-GlcpNAc-(1 --> 6)-alpha-D-Manp-(1 --> 6)-beta-D-Manp-OR (3), where R = (CH2)8COOMe, have been prepared by using combined chemical and enzymic procedures. Structure 2 is a substrate for UDP-GlcpNAc:beta-D-Galp-(1 --> 4)-beta-D-GlcpNAc (GlcNAc to Gal) beta(1 --> 3)-N-acetylglucosaminyltransferase, and 3 would be the product of its action. Antibodies raised against 3 are intended for use in an ELISA assay that would quantitate the enzymic conversion of immobilized 2 into 3.

引用

页码：83 / 93

页数：11

共 37 条

[1] CODING AND CLASSIFICATION OF D-GALACTOSE, N-ACETYL-D-GALACTOSAMINE, AND BETA-D-GALP-[1-]3(4)]-BETA-D-GLCPNAC, SPECIFICITIES OF APPLIED LECTINS
WU, AM
SUGII, S
[J]. CARBOHYDRATE RESEARCH, 1991, 213 : 127 - 143
[2] PARTIALLY BENZYLATED OXAZOLINE DERIVATIVES OF "2-ACETAMIDO-2-DEOXY-D-GLUCOPYRANOSE AS STANDARDIZED INTERMEDIATES FOR OLIGOSACCHARIDE SYNTHESIS - PREPARATION OF DISACCHARIDES HAVING THE SEQUENCES BETA-D-GLCPNAC(1-]X)-D-GAL AND BETA-D-GLCPNAC(1-]4)-D-GLCNAC
NASHED, MA
KISO, M
SLIFE, CW
ANDERSON, L
[J]. CARBOHYDRATE RESEARCH, 1981, 90 (01) : 71 - 82
[3] H-1-NMR ANALYSIS OF TYPE-2 CHAIN LACTO-GANGLIOSIDES - CONFIRMATION OF STRUCTURE OF A NOVEL CANCER-ASSOCIATED FUCOGANGLIOSIDE, ALPHA-NEUAC-(2-]6)-BETA-D-GALP-(1-]4)-BETA-D-GLCPNAC-(1-]3)-BETA-D-GALP-(1-]4)-[ALPHA-L-FUCP-(1-]3)]-BETA-D-GLCPNAC-(1-]3)-BETA-D-GALP-(1-]4)-BETA-D-GLCP-(1-]1)-CER (VI6NEUACIII3FUCNLC6CER)-
LEVERY, SB
NUDELMAN, E
KANNAGI, R
SYMINGTON, FW
ANDERSEN, NH
CLAUSEN, H
BALDWIN, M
HAKOMORI, SI
[J]. CARBOHYDRATE RESEARCH, 1988, 178 : 121 - 144
[4] SYNTHETIC STUDIES IN CARBOHYDRATES .83. SYNTHESIS OF OLIGOSACCHARIDES CONTAINING THE X-ANTIGENIC TRISACCHARIDE (ALPHA-L-FUCP-(1-]3)-[BETA-D-GALP-(1-]4)]-BETA-D-GLCPNAC) AT THEIR NONREDUCING ENDS
JAIN, RK
MATTA, KL
[J]. CARBOHYDRATE RESEARCH, 1992, 226 (01) : 91 - 100
[5] ENZYMIC-SYNTHESIS OF THE SIALYLGLYCOPEPTIDE, ALPHA-D-NEUPAC-(2-]6)-BETA-D-GALP-(1-]4)-BETA-D-GLCPNAC-(1-]4N)-L-ASN
AUGE, C
GAUTHERON, C
PORA, H
[J]. CARBOHYDRATE RESEARCH, 1989, 193 : 288 - 293
[6] H-1 and C-13 NMR analysis of the pentasaccharide Gal beta(1->4)GlcNAc beta(1->3)-[GlcNAc beta(1->6)]Gal beta(1->4)GlcNAc synthesized by the mid-chain beta-(1->6)-D-N-acetylglucosaminyltransferase of rat serum
Maaheimo, H
Rabina, J
Renkonen, O
[J]. CARBOHYDRATE RESEARCH, 1997, 297 (02) : 145 - 151
[7] Site-directed enzymatic alpha-(1->3)-L-fucosylation of the tetrasaccharide Gal beta(1->4)GlcNAc beta(1->3)Gal beta(1->4)GlcNAc at the distal N-acetyllactosamine unit
Niemela, R
Rabina, J
Leppanen, A
Maaheimo, H
Costello, CE
Renkonen, O
[J]. CARBOHYDRATE RESEARCH, 1995, 279 : 331 - 338
[8] SYNTHESIS OF THE 3''-SULFATE ESTER OF BETA-D-GALPNAC-(1-]4)-BETA-D-GLCPNAC-(1-]2)-ALPHA-D-MANP
SRIVASTAVA, V
HINDSGAUL, O
[J]. CARBOHYDRATE RESEARCH, 1989, 185 (01) : 163 - 169
[9] SYNTHETIC STUDIES ON OLIGOSACCHARIDE OF A GLYCOLIPID FROM THE SPERMATOZOA OF BIVALVES .6. SYNTHESIS OF A PRECURSOR OF BETA-D-GLCPNAC(1-]2)-ALPHA-D-MANP-(1-]3)-[BETA-D-XYLP-(1-]2)]-BETA-D-MANP-(1-]4)-BETA-D-GLCP
KANIE, O
TAKEDA, T
HADA, N
OGIHARA, Y
[J]. JOURNAL OF CARBOHYDRATE CHEMISTRY, 1991, 10 (04) : 561 - 581
[10] ACCEPTOR SUBSTRATE RECOGNITION BY N-ACETYLGLUCOSAMINYLTRANSFERASE-V - CRITICAL ROLE OF THE 4''-HYDROXYL GROUP IN BETA-D-GLCPNAC-(1-)2)-ALPHA-D-MANP(1-)6)-BETA-D-GLCP-OR
KANIE, O
CRAWLEY, SC
PALCIC, MM
HINDSGAUL, O
[J]. CARBOHYDRATE RESEARCH, 1993, 243 (01) : 139 - 164

← 1 2 3 4 →