H-1 and C-13 NMR analysis of the pentasaccharide Gal beta(1->4)GlcNAc beta(1->3)-[GlcNAc beta(1->6)]Gal beta(1->4)GlcNAc synthesized by the mid-chain beta-(1->6)-D-N-acetylglucosaminyltransferase of rat serum

被引：18
|
作者：
Maaheimo, H
Rabina, J
Renkonen, O
机构：
[1] UNIV HELSINKI,INST BIOTECHNOL,FIN-00014 HELSINKI,FINLAND
[2] UNIV HELSINKI,DIV BIOCHEM,FIN-00014 HELSINKI,FINLAND
来源：
CARBOHYDRATE RESEARCH | 1997年 / 297卷 / 02期
基金：
芬兰科学院;
关键词：
beta-(1->6)-D-N-acetylglucosaminyltransferase (GlcNAc to Gal); oligo-(N-acetyllactosamino)glycans; mid-chain branching; NMR;
D O I：
10.1016/S0008-6215(96)00259-5
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Chemical shifts and coupling constants of completely assigned H-1 and C-13 NMR spectra at 500 MHz, as well as ROESY and HMBC connectivities were used to establish the structure of the pentasaccharide Gal beta(1 --> 4)GlcNAc beta(1 --> 3)[GlcNAc beta(1 --> 6)]Gal beta(1 --> 4)GlcNAc, synthesized by the action of the mid-chain beta-(1 --> 6)-D-N-acetylglucosaminyltransferase of rat serum from UDP-GlcNAc and the linear tetrasaccharide Gal beta(1 --> 4)GlcNAc beta(1 --> 3)Gal beta(1 --> 4)GlcNAc. (C) 1997 Elsevier Science Ltd.
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页码：145 / 151
页数：7
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