FAST OLIGONUCLEOTIDE DEPROTECTION PHOSPHORAMIDITE CHEMISTRY FOR DNA-SYNTHESIS

被引：79

作者：

VU, H ^{[1
]}

MCCOLLUM, C ^{[1
]}

JACOBSON, K ^{[1
]}

THEISEN, P ^{[1
]}

VINAYAK, R ^{[1
]}

SPIESS, E ^{[1
]}

ANDRUS, A ^{[1
]}

机构：

[1] APPL BIOSYST INC,850 LINCOLN CTR DR,FOSTER CITY,CA 94404

来源：

TETRAHEDRON LETTERS | 1990年 / 31卷 / 50期

关键词：

D O I：

10.1016/S0040-4039(00)88541-X

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

A new set of base protecting groups for cyanoethylphosphoramidite nucleosides and supports has been developed which decreases the post-synthesis time requirements. The traditional purine amide protecting groups, which require 8-16 hours at 55°C for deprotection in ammonia, have been replaced with the dimethylformamidine group. Oligonucleotides made with the new reagents require only 1 hour at 55°C or 8 hours at room temperature for complete deprotection. Dialkylformamidine phosphoramidites exhibit enhanced resistance to depurination compared to the traditional, or even the phenoxyacetyl, phosphoramidites. © 1990.

引用

页码：7269 / 7272

页数：4

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