EFFICIENT SPLICING OF AN AU-RICH ANTISENSE INTRON SEQUENCE

被引：32
|
作者：
SIMPSON, CG
BROWN, JWS
机构：
[1] Cell and Molecular Genetics Department, Scottish Crop Research Institute, Dundee, DD2 5DA, Invergowrie
来源：
PLANT MOLECULAR BIOLOGY | 1993年 / 21卷 / 02期
关键词：
AU-RICH SEQUENCES; INTRON; PRE-MESSENGER-RNA SPLICING; REVERSE TRANSCRIPTASE-PCR; SPLICE SITE; TRANSIENT EXPRESSION;
D O I：
10.1007/BF00019937
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
For successful splicing in dicot plants the only recognised intron requirements are 5' and 3' splice sites and AU-rich sequences. We have investigated further the importance of AU-rich elements by analyzing the splicing of an AU-rich antisense intron sequence. Activation of cryptic splice sites on either side of the AU-rich sequence permitted the efficient removal of this essentially non-intron sequence by splicing. This splicing event not only confirms the importance of AU-rich sequences but also has implications for the evolution of interrupted genes and the expression of heterologous genes in transgenic plants.
引用
收藏
页码：205 / 211
页数：7
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