SINGLE-STRAND CONFORMATION POLYMORPHISM ANALYSIS BY PERPENDICULAR TEMPERATURE-GRADIENT GEL-ELECTROPHORESIS

被引：11
|
作者：
SUGANO, K
FUKAYAMA, N
OHKURA, H
SHIMOSATO, Y
YAMADA, Y
INOUE, T
SEKIYA, T
HAYASHI, K
机构：
[1] NATL CANC CTR,DIV MED ONCOL,TOKYO,JAPAN
[2] NATL CANC CTR,RES INST,DIV ONCOGENE,TOKYO 104,JAPAN
[3] KITAZATO SUPPLY CO,FUJINOMIYA,JAPAN
[4] KYUSHU UNIV,INST GENET INFORMAT,DIV GENOME ANAL,FUKUOKA 812,JAPAN
来源：
ELECTROPHORESIS | 1995年 / 16卷 / 01期
关键词：
POLYMERASE CHAIN REACTION; SINGLE-STRAND CONFORMATION POLYMORPHISM; TEMPERATURE-GRADIENT GEL ELECTROPHORESIS; K-RAS; P53;
D O I：
10.1002/elps.1150160103
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Using a newly developed temperature-gradient gel electrophoresis apparatus, mutations in the K-ras oncogene and p53 tumor supressor gene were analyzed for single-strand conformation polymorphism (SSCP). The mobilities of single-stranded DNAs, carrying various mutations, change - depending on the gel temperature during electrophoresis. Therefore, temperature-gradient single-strand conformation polymorphism (TG-SSCP) analysis can provide useful information concerning the optimum temperature for SSCP and may also be used to screen for various mutations or polymorphisms in a single electrophoretic run.
引用
收藏
页码：8 / 10
页数：3
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