SINGLE-STRAND CONFORMATION POLYMORPHISM ANALYSIS BY PERPENDICULAR TEMPERATURE-GRADIENT GEL-ELECTROPHORESIS

被引：11

作者：

SUGANO, K

FUKAYAMA, N

OHKURA, H

SHIMOSATO, Y

YAMADA, Y

INOUE, T

SEKIYA, T

HAYASHI, K

机构：

[1] NATL CANC CTR,DIV MED ONCOL,TOKYO,JAPAN

[2] NATL CANC CTR,RES INST,DIV ONCOGENE,TOKYO 104,JAPAN

[3] KITAZATO SUPPLY CO,FUJINOMIYA,JAPAN

[4] KYUSHU UNIV,INST GENET INFORMAT,DIV GENOME ANAL,FUKUOKA 812,JAPAN

来源：

ELECTROPHORESIS | 1995年 / 16卷 / 01期

关键词：

POLYMERASE CHAIN REACTION; SINGLE-STRAND CONFORMATION POLYMORPHISM; TEMPERATURE-GRADIENT GEL ELECTROPHORESIS; K-RAS; P53;

D O I：

10.1002/elps.1150160103

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Using a newly developed temperature-gradient gel electrophoresis apparatus, mutations in the K-ras oncogene and p53 tumor supressor gene were analyzed for single-strand conformation polymorphism (SSCP). The mobilities of single-stranded DNAs, carrying various mutations, change - depending on the gel temperature during electrophoresis. Therefore, temperature-gradient single-strand conformation polymorphism (TG-SSCP) analysis can provide useful information concerning the optimum temperature for SSCP and may also be used to screen for various mutations or polymorphisms in a single electrophoretic run.

引用

页码：8 / 10

页数：3

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