ANTI-HBS DETECTION - A MODIFIED PASSIVE HEMAGGLUTINATION ASSAY

被引：0

作者：

MCDONALD, CP ^{[1
]}

BARBARA, JAJ ^{[1
]}

ALIZZI, A ^{[1
]}

AHYA, R ^{[1
]}

VYAS, N ^{[1
]}

CONTRERAS, M ^{[1
]}

机构：

[1] ROYAL FREE HOSP,LONDON NW3 2QG,ENGLAND

来源：

MEDICAL LABORATORY SCIENCES | 1992年 / 49卷 / 01期

关键词：

HEMAGGLUTINATION; HEPATITIS-B ANTIBODIES;

D O I：

暂无

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Modification of a commercial haemagglutination assay for the detection of anti-HBs has reduced the test time from over one hour to approximately 25 min, increased sensitivity ten-fold without any prozoning, maintained specificity and reduced costs by 90%. The modification consists of diluting the reagent cells ten-fold; these are then added to dilutions of test serum in a V-well microplate. After incubation, plates are centrifuged and then inclined at 70-degrees. Positive and negative reactions can be clearly distinguished within approximately 10 minutes.

引用

页码：66 / 69

页数：4

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