THE KINETICS OF RCC1 INCLUSION-BODY FORMATION IN ESCHERICHIA-COLI

被引：4
|
作者：
TSAI, AM
BETENBAUGH, MJ
SHILOACH, J
机构：
[1] NIDDK,BIOTECHNOL UNIT,BETHESDA,MD 20892
[2] JOHNS HOPKINS UNIV,DEPT CHEM ENGN,BALTIMORE,MD 21218
来源：
BIOTECHNOLOGY AND BIOENGINEERING | 1995年 / 48卷 / 06期
关键词：
CHROMOSOME CONDENSATION PROTEIN; INCLUSION BODY; ESCHERICHIA COLI;
D O I：
10.1002/bit.260480620
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The Regulator of Chromosome Condensation protein (RCC1) is located in both the soluble and inclusion body (IB) fractions of the whole cell lysate when expressed in Escherichia coli BL21 (pLysS) at temperatures below 30 degrees C. When bacterial growth was carried out at 20 degrees C, the majority of the RCC1 remained soluble up to 5.5 h postinduction. When the temperature was raised to 25 degrees C, RCC1 IB was dominant by 1.5 h postinduction. The shift in RCC1 IB formation with temperature suggests that in addition to increased translation rates, folding and aggregation processes may contribute to RCC1 IB formation at higher temperatures. (C) 1995 John Wiley & Sons, Inc.
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收藏
页码：715 / 718
页数：4
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