LYMPHOCYTAPHERESIS IN COMBINATION WITH IMMUNOSUPPRESSIVE DRUGS FOR REFRACTORY MYASTHENIA-GRAVIS - 2-COLOR FLOW CYTOMETRIC ANALYSIS OF CHANGES IN PERIPHERAL-BLOOD LYMPHOCYTE SUBSETS

We carried out lymphocytapheresis (LCP) in combination with the administration of immunosuppressive drugs in patients with myasthenia gravis (MG), who were resistant to conventional immunosuppressive therapy, and examined its efficacy and effects on peripheral blood lymphocyte subsets. LCP was carried out once a week for 1 month (one course, 4 times) using a continuous-flow blood cell separator. Immunosuppressive medication (prednisolone or prednisolone and azathioprine) was continued during the course of treatment. After LCP, clinical improvement was noted in 5 of 6 patients. Anti-AChR antibody titers and the number of lymphocytes were significantly reduced in all patients. A significant decrease in CD4(+)CD45RA(-) (memory) T cell level and significant increase in CD4(+)CD45RA(+) (naive) T cell level were also observed. In the patients having good response to LCP, follow-up evaluation showed long-term clinical improvements, as well as the memory T cell level staying at the decreased level. Our study suggests that LCP in combination with the administration of immunosuppressive drugs can suppress the disease activity of MG.