CHARACTERIZATION OF 5-HT3 RECEPTORS IN INTACT N1E-115 NEUROBLASTOMA-CELLS

被引：47
|
作者：
LUMMIS, SCR
KILPATRICK, GJ
MARTIN, IL
机构：
[1] MRC,MOLEC NEUROBIOL UNIT,CAMBRIDGE CB2 2QH,ENGLAND
[2] GLAXO GRP RES LTD,DEPT NEUROPHARMACOL,WARE SG12 0DP,HERTS,ENGLAND
来源：
EUROPEAN JOURNAL OF PHARMACOLOGY-MOLECULAR PHARMACOLOGY SECTION | 1990年 / 189卷 / 2-3期
关键词：
5-HT3; RECEPTOR; N1E-115 NEUROBLASTOMA CELLS; GR65630;
D O I：
10.1016/0922-4106(90)90026-T
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
The highly selective 5-HT3 receptor antagonist [H-3]GR65630 has been used to characterize 5-HT3 receptors in intact N1E-115 neuroblastoma cells. Equilibrium binding analysis demonstrated high-affinity binding to a single class of receptors with a K(d) of 0.69 (+/- 0.12) nM and B(max) of 31.4 (+/- 11.4) fmol/10(5) cells, equivalent to approximately 200 000 sites per cell. Specific binding was displaced by low concentrations of 5-HT3-selective ligands, and by the nicotinic antagonist d-tubocurarine.
引用
收藏
页码：223 / 227
页数：5
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