CHARACTERIZATION OF 5-HT3 RECEPTORS IN INTACT N1E-115 NEUROBLASTOMA-CELLS

被引：47
|
作者：
LUMMIS, SCR
KILPATRICK, GJ
MARTIN, IL
机构：
[1] MRC,MOLEC NEUROBIOL UNIT,CAMBRIDGE CB2 2QH,ENGLAND
[2] GLAXO GRP RES LTD,DEPT NEUROPHARMACOL,WARE SG12 0DP,HERTS,ENGLAND
来源：
EUROPEAN JOURNAL OF PHARMACOLOGY-MOLECULAR PHARMACOLOGY SECTION | 1990年 / 189卷 / 2-3期
关键词：
5-HT3; RECEPTOR; N1E-115 NEUROBLASTOMA CELLS; GR65630;
D O I：
10.1016/0922-4106(90)90026-T
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
The highly selective 5-HT3 receptor antagonist [H-3]GR65630 has been used to characterize 5-HT3 receptors in intact N1E-115 neuroblastoma cells. Equilibrium binding analysis demonstrated high-affinity binding to a single class of receptors with a K(d) of 0.69 (+/- 0.12) nM and B(max) of 31.4 (+/- 11.4) fmol/10(5) cells, equivalent to approximately 200 000 sites per cell. Specific binding was displaced by low concentrations of 5-HT3-selective ligands, and by the nicotinic antagonist d-tubocurarine.
引用
收藏
页码：223 / 227
页数：5
相关论文
共 50 条
  • [1] THE ANTAGONIST PROPERTIES OF S 11978 ON 5-HT3 RECEPTORS IN N1E-115 NEUROBLASTOMA-CELLS
    MORAIN, P
    ABRAHAM, C
    LACOSTE, JM
    MALEN, C
    LAUBIE, M
    GIESENCROUSE, E
    [J]. JOURNAL OF NEUROCHEMISTRY, 1993, 61 : S91 - S91
  • [2] CHLORPROMAZINE AND QX222 BLOCK 5-HT3, RECEPTORS IN N1E-115 NEUROBLASTOMA-CELLS
    SEPULVEDA, MI
    BAKER, J
    LUMMIS, SCR
    [J]. NEUROPHARMACOLOGY, 1994, 33 (3-4) : 493 - 499
  • [3] TARGET SIZE OF 5-HT3 RECEPTORS IN N1E-115 NEUROBLASTOMA-CELLS AND RAT-BRAIN
    LUMMIS, SCR
    NIELSEN, M
    KILPATRICK, GJ
    MARTIN, IL
    [J]. EUROPEAN JOURNAL OF PHARMACOLOGY-MOLECULAR PHARMACOLOGY SECTION, 1990, 189 (2-3): : 229 - 232
  • [4] COMPETITIVE AND NONCOMPETITIVE EFFECTS OF 5-HYDROXYINDOLE ON 5-HT3 RECEPTORS IN N1E-115 NEUROBLASTOMA-CELLS
    KOOYMAN, AR
    VANHOOFT, JA
    VANDERHEIJDEN, PML
    VIJVERBERG, HPM
    [J]. BRITISH JOURNAL OF PHARMACOLOGY, 1994, 112 (02) : 541 - 546
  • [5] Actions of general anaesthetics on 5-HT3 receptors in N1E-115 neuroblastoma cells
    Jenkins, A
    Franks, NP
    Lieb, WR
    [J]. BRITISH JOURNAL OF PHARMACOLOGY, 1996, 117 (07) : 1507 - 1515
  • [6] CHARACTERIZATION OF 5-HT3 RECEPTORS OF N1E-115 NEUROBLASTOMA-CELLS BY USE OF THE INFLUX OF THE ORGANIC CATION [C-14] GUANIDINIUM
    BONISCH, H
    BARANN, M
    GRAUPNER, J
    GOTHERT, M
    [J]. BRITISH JOURNAL OF PHARMACOLOGY, 1993, 108 (02) : 436 - 442
  • [7] CLONING AND FUNCTIONAL EXPRESSION OF A 5-HT3 RECEPTOR CDNA FROM N1E-115 NEUROBLASTOMA-CELLS
    HOPE, AG
    DOWNIE, DL
    LAMBERT, JJ
    PETERS, JA
    SUTHERLAND, L
    BURCHELL, B
    [J]. BRITISH JOURNAL OF PHARMACOLOGY, 1993, 108 : P103 - P103
  • [8] ANTAGONISM OF 5-HT3 RECEPTOR MEDIATED CURRENTS IN MURINE N1E-115 NEUROBLASTOMA-CELLS BY (+)-TUBOCURARINE
    PETERS, JA
    MALONE, HM
    LAMBERT, JJ
    [J]. NEUROSCIENCE LETTERS, 1990, 110 (1-2) : 107 - 112
  • [9] THE AGONIST PROPERTIES OF META-CHLOROPHENYLBIGUANIDE AND 2-METHYL-5-HYDROXYTRYPTAMINE ON 5-HT3 RECEPTORS IN N1E-115 NEUROBLASTOMA-CELLS
    SEPULVEDA, MI
    LUMMIS, SCR
    MARTIN, IL
    [J]. BRITISH JOURNAL OF PHARMACOLOGY, 1991, 104 (02) : 536 - 540
  • [10] THE ANTAGONIST PROPERTIES OF S-11978 ON 5HT3 RECEPTORS IN N1E-115 NEUROBLASTOMA-CELLS
    MORAIN, P
    ABRAHAM, C
    LACOSTE, JM
    MALEN, C
    LAUBIE, M
    GIESENCROUSE, E
    [J]. FUNDAMENTAL & CLINICAL PHARMACOLOGY, 1993, 7 (05) : 205 - 208
12345