AROMATASE-ACTIVITY AND THE EFFECT OF ESTRADIOL AND TESTOSTERONE ON DNA-SYNTHESIS IN ENDOMETRIAL CARCINOMA CELL-LINES

被引：23

作者：

TADA, A ^{[1
]}

SASAKI, H ^{[1
]}

NAKAMURA, J ^{[1
]}

YOSHIHAMA, M ^{[1
]}

TERASHIMA, Y ^{[1
]}

机构：

[1] SNOW BRAND MILK PROD CO,LIFE SCI RES INST,TOCHIGI,JAPAN

来源：

JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY | 1993年 / 44卷 / 4-6期

关键词：

D O I：

10.1016/0960-0760(93)90276-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Human endometrial and breast carcinoma cell lines were examined for aromatase activity and the effects of sex steroids (estradiol and testosterone) on DNA synthesis. Aromatase activity was high (greater than 500 fmol/10(7) cells/24 h) in the cell lines MCF-7 and OMC-2, moderate (100-499 fmol/10(7) cells/24 h) in the cell lines HEC-59 and Ishikawa, and low (less than 100 fmol/10(7) cells/24 h) in the HHUA cell line. A substantial stimulation of DNA synthesis by estradiol (10(-9) M) was observed in cell lines HEC-59, OMC-2, and MCF-7, with an increase in [H-3]thymidine uptake of over 250%. The Ishikawa cell line was stimulated moderately (115-249%). No estradiol-induced increase in DNA synthesis was observed in HHUA. Responsiveness of DNA synthesis to testosterone was observed in cell lines that showed the greatest response to estradiol, namely HEC-59, OMC-2, and MCF-7. Otherwise, estrogen-responsiveness did not always correlate with a significant aromatase activity. These data suggest that some but not all endometrial carcinomas may possess an aromatase-dependent growth stimulating system.

引用

页码：661 / 666

页数：6

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