DEVELOPMENT OF A PCR ASSAY COMBINED WITH A SHORT ENRICHMENT CULTURE FOR DETECTION OF CAMPYLOBACTER-JEJUNI IN ESTUARINE SURFACE WATERS

被引：0
|
作者：
HERNANDEZ, J
ALONSO, JL
FAYOS, A
AMOROS, I
OWEN, RJ
机构：
[1] UNIV POLITECN VALENCIA,INST HIDROL & MED NAT,E-46022 VALENCIA,SPAIN
[2] CENT PUBL HLTH LAB,NATL COLLECT TYPE CULTURES,LONDON NW9 5HT,ENGLAND
来源：
FEMS MICROBIOLOGY LETTERS | 1995年 / 127卷 / 03期
关键词：
CAMPYLOBACTER JEJUNI; POLYMERASE CHAIN REACTION; DNA; SURFACE WATER;
D O I：
暂无
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Two extraction procedures were examined, and it was found that DNA recovered from Campylobacter jejuni lysed by the cetyltrimethylammonium bromide (CTAB) method was more suitable for use as a PCR template than DNA released by the boiling method. The region targeted for PCR amplification was a 1.73-kb portion of the flagellin A gene of C. jejuni. The detection limit was lower than 30 cells per 100 ml in artificially contaminated waters. PCR assay and conventional culturing method had the same sensitivity, but results of the PCR technique were available within 48 h and so shortened the time necessary for detection by 48 h.
引用
收藏
页码：201 / 206
页数：6
相关论文
共 50 条
  • [21] A real-time multiplexed PCR assay for rapid detection and differentiation of Campylobacter jejuni and Campylobacter coli
    LaGier, MJ
    Joseph, LA
    Passaretti, TV
    Musser, KA
    Cirino, NA
    [J]. MOLECULAR AND CELLULAR PROBES, 2004, 18 (04) : 275 - 282
  • [22] Development of a combined filtration-enrichment culture followed by a one-step duplex PCR technique for the rapid detection of Campylobacter jejuni and C-coli in human faecal samples
    Misawa, N
    Kawashima, K
    Kawamoto, H
    Kondo, F
    [J]. JOURNAL OF MEDICAL MICROBIOLOGY, 2002, 51 (01) : 86 - 89
  • [23] Development and evaluation of internal amplification controls for use in a real-time duplex PCR assay for detection of Campylobacter coli and Campylobacter jejuni
    Randall, Luke
    Lemma, Fabrizio
    Rodgers, John
    Vidal, Ana
    Clifton-Hadley, Felicity
    [J]. JOURNAL OF MEDICAL MICROBIOLOGY, 2010, 59 (02) : 172 - 178
  • [24] Development and evaluation of aptamer magnetic capture assay in conjunction with real-time PCR for detection of Campylobacter jejuni
    Suh, Soo Hwan
    Dwivedi, Had P.
    Jaykus, Lee-Ann
    [J]. LWT-FOOD SCIENCE AND TECHNOLOGY, 2014, 56 (02) : 256 - 260
  • [25] A survey on the incidence of Campylobacter spp. and the development of a surface adhesion polymerase chain reaction (SA-PCR) assay for the detection of Campylobacter jejuni in retail meat products
    Cloak, OM
    Duffy, G
    Sheridan, JJ
    Blair, IS
    McDowell, DA
    [J]. FOOD MICROBIOLOGY, 2001, 18 (03) : 287 - 298
  • [26] A Charcoal- and Blood-Free Enrichment Broth for Isolation and PCR Detection of Campylobacter jejuni and Campylobacter coli in Chicken
    Solis-Soto, Luisa Y.
    Garcia, Santos
    Wesley, Irene
    Heredia, Norma
    [J]. JOURNAL OF FOOD PROTECTION, 2011, 74 (02) : 221 - 227
  • [27] Development of a quantification method for small numbers of Campylobacter jejuni by combining real time PCR and enrichment
    Shahada, F.
    Nomoto, Y.
    Chuma, T.
    Okamoto, K.
    Yamamoto, S.
    [J]. ZOONOSES AND PUBLIC HEALTH, 2007, 54 : 26 - 27
  • [28] Development of a multiplex PCR assay for identification of Campylobacter coli, Campylobacter fetus, Campylobacter hyointestinalis subsp hyointestinalis, Campylobacter jejuni, Campylobacter lari and Campylobacter upsaliensis
    Yamazaki-Matsune, Wataru
    Taguchi, Masurni
    Seto, Kazuko
    Kawahara, Ryuji
    Kawatsu, Kentaro
    Kumeda, Yuko
    Kitazato, Miyoshi
    Nukina, Masafumi
    Misawa, Naoaki
    Tsukamoto, Teizo
    [J]. JOURNAL OF MEDICAL MICROBIOLOGY, 2007, 56 (11) : 1467 - 1473
  • [29] Development of a ceuE-based multiplex polymerase chain reaction (PCR) assay for direct detection and differentiation of Campylobacter jejuni and Campylobacter coli in Thailand
    Houng, HSH
    Sethabutr, O
    Nirdnoy, W
    Katz, DE
    Pang, LW
    [J]. DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2001, 40 (1-2) : 11 - 19
  • [30] Use of culture, PCR analysis, and DNA microarrays for detection of Campylobacter jejuni and Campylobacter coli from chicken feces
    Keramas, G
    Bang, DD
    Lund, M
    Madsen, M
    Bunkenborg, H
    Telleman, P
    Christensen, CBV
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2004, 42 (09) : 3985 - 3991
12345