CONDITIONAL GROWTH OF ESCHERICHIA-COLI CAUSED BY EXPRESSION OF VACCINIA VIRUS-DNA TOPOISOMERASE-I

被引：13
|
作者：
FERNANDEZBEROS, ME [1 ]
TSEDINH, YC [1 ]
机构：
[1] NEW YORK MED COLL,DEPT BIOCHEM & MOLEC BIOL,VALHALLA,NY 10595
来源：
JOURNAL OF BACTERIOLOGY | 1992年 / 174卷 / 21期
关键词：
D O I：
10.1128/JB.174.21.7059-7062.1992
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Active vaccinia virus topoisomerase I is expressed in Escherichia coli containing plasmid p1940 (S. Shuman, M. Golder, and B. Moss, J. Biol. Chem. 263:16401-16407, 1988). Growth curves showed a decline of 2 to 3 logs in the number of viable cells at 42-degrees-C after shift from 30-degrees-C because of increased vaccinia virus topoisomerase I level. Mutations in the gyrA and gyrB genes allowed cells to grow equally well at 42 and 30-degrees-C. The presence of gyrase inhibitor also improved growth at 42-degrees-C.
引用
收藏
页码：7059 / 7062
页数：4
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