THE CHARACTERIZATION OF THE O-ANTIGEN OF ESCHERICHIA-COLI 064-K99 LIPOPOLYSACCHARIDE

被引：6
|
作者：
PERRY, MB
MACLEAN, LL
BRISSON, JR
机构：
来源：
CARBOHYDRATE RESEARCH | 1993年 / 248卷
关键词：
D O I：
10.1016/0008-6215(93)84134-R
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The structure of the O-polysaccharide of the smooth lipopolysaccharide (LPS) produced by Escherichia coli O64:K99 was investigated by SDS-PAGE, composition, periodate oxidation, methylation, partial hydrolysis, and 1D and 2D nuclear magnetic resonance analyses, made on the native o-chain and its reduction and periodate degradation products. The E. coli O64 antigenic O-chain was found to be a high molecular weight glycan composed of D-galactose, D-glucuronic acid, 2-acetamido-2-deoxy-D-glucose, and 2-acetamido-2-deoxy-D-mannose (2:1:1:1) and was a polymer of branched pentasaccharide repeating units having the structure: [GRAPHICS].
引用
收藏
页码：277 / 284
页数：8
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