IDENTIFICATION OF POLYMORPHISM IN LEPTIN GENE OF MURRAH BUFFALO BY PCR-RFLP

Present study was undertaken with the objective to identify the genetic variation in exon 2 and exon 3 of leptin gene and to determine the association of variants with milk production and fat% in Murrah buffaloes. Genomic DNA was isolated from 150 lactating Murrah buffaloes. The targeted segment was amplified by PCR using oligonucleotide primers and respective amplicons of 289 bp and 405 bp size comprising of exon 2 and exon 3 were obtained. PCR-RFLP was carried out using a battery of restriction endonucleases viz. AluI, AciI, MspI, PvuII, HindIII, HinfI, Eco32I, Eco147I and Kpn2I to explore genetic variation. Data were recorded pertaining to parity-wise milk production and fat%. Results indicated monomorphic patterns of both the exons of leptin gene in Murrah buffalo. Data on milk yield and fat percentage were recorded. However, association study was not possible due to monomorphic exhibition of the gene in all the animals included in the study.