ASPECTS REGARDING THE PROFILE OF INTESTINAL MICROBIOTA ON WILD POPULATIONS OF STERLET (ACIPENSER RUTHENUS, LINNAEUS, 1758)

被引：0

作者：

Bacanu, Manuela Gianina ^{[1
]}

Oprea, Lucian ^{[1
]}

Sandu, Georgiana Calin ^{[1
]}

Dinica, Rodica ^{[1
]}

Maereanu, Marilena ^{[2
]}

Maereanu, Dumitru ^{[2
]}

Serban, Constanta ^{[3
]}

机构：

[1] Dunarea Jos Univ Galati, Fac Food Sci & Engn, 111 Domneasca St, Galati, Romania

[2] Kaviar House Bucuresti Filiala Tulcea, Isaccea, Romania

[3] ITCSMS Braila, Galati, Romania

来源：

ANNALS OF THE UNIVERSITY DUNAREA DE JOS OF GALATI, FASCICLE VI-FOOD TECHNOLOGY | 2012年 / 36卷 / 02期

关键词：

bacterial community; sterlet; PCR; DGGE;

D O I：

暂无

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

The aim of the present research was to examinate the profile of intestinal microbiota of sterlet from the Danube River. Genomic DNA was extracted from each gut fish and polymerase chain reaction (PCR) was used to amplify the conserved 16S ribosomal RNA gene. Using Denaturing Gradient Gel Electrophoresis (DGGE) of PCR amplified 16S rDNA to characterise the variability of bacterial populations, the results showed different microbial profiles for 50% of fish. These results demonstrate the potential of PCRDGGE- based analysis for identification of gut microbiota and could contribute to a better understanding of fish ecology.

引用

页码：58 / 63

页数：6

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